Two-Tailed RT-qPCR technology
Patented Two-Tailed RT-qPCR technology with a unique mechanism of RT-primers. Two-Tailed RT-qPCR is standardized for miRNA and piRNA total quantification. MiRXES assays are designed in silico using proprietary 30-parameter thermodynamics based algorithms and are extensively validated using both synthetic miRNAs and RNA from biological samples.
- Manual for TT-RT-qPCR kits
- RNA Isolation Kit
- microRNAs for Diagnostics in Cancer
- microRNAs for Diagnostics in Cardiovascular Disease
- microRNAs for Diagnostics in Colorectal Cancer
- microRNAs for Diagnostics in Metabolic Syndrome
miREIA – a immunoassay-based method of miRNA quantification
miREIA is a novel immunoassay-based method of miRNA quantification which involves hybridization of miRNA isolated from a patient sample to complementary biotinylated DNA oligonucleotide probe. The DNA/RNA hybrids are then captured by a microtiter plate-immobilized monoclonal antibody specific to perfectly matched DNA/miRNA hybrids. Next steps are copying standard ELISA protocols.
The assay can be run on common immunoassay equipment, is compatible with standard clinical workflow, does not require amplification steps and results are obtained in less than three hours including miRNA profiling.